منابع مشابه
Genetic screens in vivo using the CRISPR / Cas 9 system
An unmet and paramount need in the field of cancer research is to rapidly translate basic biological findings to clinically relevant therapeutics for cancer patients. Recent technological advances have generated many innovative applications to cancer biology and in a short time have yielded a wealth of information about putative vulnerabilities across a range of cancers. The proposed work invol...
متن کاملGenome-scale CRISPR pooled screens.
Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review...
متن کاملGenetic screens in human cells using the CRISPR-Cas9 system.
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system for genome editing has greatly expanded the toolbox for mammalian genetics, enabling the rapid generation of isogenic cell lines and mice with modified alleles. Here, we describe a pooled, loss-of-function genetic screening approach suitable for both positive and negative selection that uses a genome-sc...
متن کامل“CRISPR” validation of recessive brain cancer genes in vivo
Profound understanding of the molecular events driving oncogenic transformation is essential for the development of improved therapies against cancer. While accumulating next generation sequencing data provide a plethora of candidate genes for various cancer entities [1], validating the oncogenic potential of these genes proved to be more challenging. Autochthonous mouse models emerged as a gol...
متن کاملDecoding directional genetic dependencies through orthogonal CRISPR/Cas screens
1. Department of Microbiology and Immunology; Diabetes Center at UCSF, WM Keck Center for Noncoding RNAs, University of California, San Francisco, San Francisco, California, USA. 2. Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, and Chan-Zuckerberg Biohub, San Francisco, California, USA. 3. Helen Diller Family Compre...
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ژورنال
عنوان ژورنال: Trends in Cancer
سال: 2018
ISSN: 2405-8033
DOI: 10.1016/j.trecan.2018.03.002